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Global Treasures Rabat Morocco
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String from the Sky
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The collection and retention of a range of common airborne spore types trapped directly into microtiter wells for enzyme-linked immunosorbent analysis [An article from: Journal of Aerosol Science]
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This digital document is a journal article from Journal of Aerosol Science, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
The collection and retention of a range of fungal spores for a novel spore trap (Microtiter immunospore trap (MTIST)), designed for use with immunoassays, was investigated in wind tunnel experiments. Concentrations of spores of Botrytis cinerea, Cladosporium cladosporioides, Lycopodium clavatum, Erysiphe cruciferarum and Penicillium roqueforti, were measured using the MTIST and miniature suction traps (same characteristics as a Burkard 7 day volumetric spore trap). A clear relationship was observed between the spore concentrations measure by the MTIST and those measured by the mini-suction traps. No significant (F"p"r"o"b>0.4) effect of windspeed was observed for any spore type in samples collected in the MTIST trap. There was some effect on windspeed in the collection of the larger spore types (Lycopodium) as measured by the mini-suction traps. Pre-coating microtiter wells of the MTIST spore trap with a mixture of paraffin wax and petroleum jelly significantly increased the collection of the two largest spore types. There were significant differences between the MTIST and the mini-suction spore traps in their ability to trap the smaller spore types (Cladosporium and Penicillium). Employing a Botrytis-specific antiserum, a high level of correlation was observed between the numbers of Botrytis conidia trapped by the MTIST spore trap and, corresponding absorbance values of an ELISA when, wells were pre-coated with either distilled water, poly-L-lysine or a petroleum jelly and paraffin wax mixture.

Fine-particle Mn and other metals linked to the introduction of MMT into gasoline in Sydney, Australia: Results of a natural experiment [An article from: Atmospheric Environment]
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This digital document is a journal article from Atmospheric Environment, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Using a combination of accelerator-based ion beam methods we have analysed PM"2"."5 particulates for a suite of 21 species (H, C, Na, Al, Si, P, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Br, Pb) to evaluate the contribution to Sydney (New South Wales, Australia) air associated with the introduction of MMT as a replacement for lead. MMT was discontinued in 2004. Teflon filters representing continuous sampling for a 7 year period from 1998 to 2004 were analysed from two sites: one from Mascot, a suburb close to the Central Business District [CBD (n=718)] and a high trafficked area, and the other, a relatively rural (background) setting at Richmond, ~20km west of the CBD (n=730). Manganese concentrations in air at the background site increased from a mean of 1.5-1.6ngm^-^3 to less than 2ngm^-^3 at the time of greatest MMT use whereas those at Mascot increased from about 2 to 5ngm^-^3. From the maximum values, the Mn showed a steady decrease at both sites concomitant with the decreasing use of MMT. Lead concentrations in air at both sites decreased from 1998 onwards, concomitant with the phase out of leaded gasoline, attained in 2002. Employing previously determined elemental signatures it was possible to adjust effects from season along with auto emissions and soil. A high correlation was obtained for the relationship between Mn in air and lead replacement gasoline use (R^2 0.83) and an improved correlation for Mn/ Al+Si+K and lead replacement gasoline use (R^2 0.93). In addition, using Mn concentrations normalized to background values of Al+Si+K or Ti to account for the lithogenically derived Mn, the proportion of anthropogenic Mn was approximately 70%. The changes for Mn and Pb detected in the particulates are attributed to the before-during-after use of MMT and decreasing use of lead in gasoline. The values measured in Sydney air are well below the reference concentration of 50ng Mnm^-^3. The incremental increases in air, however, are larger than expected given the limited use of MMT only in lead replacement gasoline and high quality monitoring should be undertaken in countries where MMT is used in all gasoline.