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SK5USP 5 Gallon UniSorb Plus Spill Kit works with acids, alkalis, solvents, oils, and water based liquids. Contains 5 gallon pail with cover, four sock (3
SK5USP 5 Gallon UniSorb Plus Spill Kit works with acids, alkalis, solvents, oils, and water based liquids. Contains 5 gallon pail with cover, four sock (3" x 48"), six pads (17" x 19"), one disposal bag and wire tie, one warning label, one Haz-mat Respons


Contents: One 5 gallon pail with cover, four socks (3" x 48"), six pads (15" x 19"), one disposal bag and wire tie, one warning label, one Haz-mat Response guidebook and Spill Kit instructions. In addition, the UniSorb Plus also contains one pair of nitri
Capillary electrophoresis for capture and concentrating of target nucleic acids by affinity gels modified to contain single-stranded nucleic acid probes [An article from: Analytica Chimica Acta]
Capillary electrophoresis for capture and concentrating of target nucleic acids by affinity gels modified to contain single-stranded nucleic acid probes [An article from: Analytica Chimica Acta]

$10.95
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Selective capture and pre-concentration of target nucleic acids from relatively complicated samples may provide a method to facilitate introduction to a microfluidic-based detection system to improve detection limits. An acrylamide polymer gel modified with Acrydite^(TM) that contained 20mer oligonucleotide probe was prepared and loaded into a capillary column. The results indicated that the amount of probe DNA that was captured into the acrylamide was about 40% of the starting monomer, and different quantities of probe could therefore be coupled into the gel. The gel was passivated by pre-treatment with non-complementary DNA oligonucleotide to block non-selective adsorption sites, and the gel was determined to be stable for multiple cycles of use. The probe could hybridize with target sequences that were introduced by electrokinetic injection from a sample solution. The target could be freed from the polymer gel by use of a combination of heating, chaotropic salt and voltage conditions. Target capture efficiency was up to 90% when using samples that did not saturate probe sites in the columns, and recovery of target from the gel could be as high as 95%.

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