antibody linked

Miniature biochip system for detection of Escherichia coli O157:H7 based on antibody-immobilized capillary reactors and enzyme-linked immunosorbent assay [An article from: Analytica Chimica Acta]
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
In this work, we report Escherichia coli O157:H7 detection using antibody-immobilized capillary reactors, enzyme-linked immunosorbent assay (ELISA), and a biochip system. ELISA selective immunological method to detect pathogenic bacteria. ELISA is also directly adaptable to a miniature biochip system that utilizes conventional sample platforms such as polymer membranes and glass. The antibody-immobilized capillary reactor is a very attractive sample platform for ELISA because of its low cost, compactness, reuse, and ease of regeneration. Moreover, an array of capillary reactors can provide high-throughput ELISA. In this report, we describe the use of an array of antibody-immobilized capillary reactors for multiplex detection of E. coli O157:H7 in our miniature biochip system. Side-entry laser beam irradiation to an array of capillary reactors contributes significantly to miniaturized optical configuration for this biochip system. The detection limits of E. coli O157:H7 using the ELISA and Cy5 label-based immunoassays were determined to be 3 and 230 cells, respectively. This system shows capability to simultaneously monitor multifunctional immunoassay and high sensitive detection of E. coli O157:H7.

Monoclonal antibody-based enzyme-linked immunosorbent assays for the detection of the organophosphorus insecticide isofenphos [An article from: Analytica Chimica Acta]
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
In our previous study, polyclonal antibodies against the organophosphorus insecticide isofenphos were generated and enzyme-linked immunosorbent assays (ELISAs) for this pesticide were developed. In this study, isofenphos ELISAs with higher performance were developed by using a monoclonal antibody and a larger number of immunoreagents. Using the antibody and a coating antigen, an indirect competitive ELISA for isofenphos was developed, which showed an IC"5"0 value of 35ng/mL with a detection limit of 5.8ng/mL. A direct competitive ELISA using an enzyme tracer was also developed, which showed an IC"5"0 value of 26ng/mL with a detection limit of 4.8ng/mL. The antibodies in both assays showed negligible cross-reactivity with other OP pesticides. Recoveries of isofenphos from fortified rice and lettuce samples ranged 30-328 and 52-197%, respectively. The bias in the recovery values was rationalized by using the standard curves obtained in the matrix extract.

Monoclonal antibody-based competitive assay for the sensitive detection of coeliac disease toxic prolamins [An article from: Analytica Chimica Acta]
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Current immunosorbent assays for gluten detection suffer from a number of drawbacks including lack of specificity (e.g. cross-reaction with coeliac disease non-toxic prolamins, detection of total gluten and not specifically coeliac disease toxic gluten) and an inability to detect quantitatively hydrolysates and prolamins present in rye, barley and, possibly, oats. Additionally, the extraction methods employed involve the use of strong reducing agents that are not compatible with enzyme-linked immunosorbent assay. This work reports on the development of a novel competition assay based on the use of a monoclonal antibody raised against a 19-mer peptide recognised in vivo to be coeliac disease toxic. This assay is specific to those cereal prolamins that exacerbate coeliac disease, is capable of the detection of hydrolysate forms and is compatible with typical extraction agents. The optimal assay format is highly sensitive with a detection limit of 0.128ppm and good reproducibility is obtained with intra-plate and inter-plate relative standard deviation of 3.21% (n=6) and 3.57% (n=3) being obtained, respectively. The reported system is the first of several systems under development that aim to address the drawbacks that current assays suffer and meet the sensitivity requirements outlined by the World Health Organisation and Codex Alimentarius.

Monoclonal antibody-based enzyme-linked immunosorbent assays for the detection of the organophosphorus insecticide diazinon [An article from: Analytica Chimica Acta]
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Four haptens of the organophosphorus (OP) insecticide diazinon were synthesized to develop enzyme-linked immunosorbent assays (ELISAs) for this pesticide. One of them was conjugated to KLH to be used as the immunogen for production of monoclonal antibodies. By using the antibodies and a coating antigen, an indirect competitive ELISA was developed, which showed an IC"5"0 of 4.0ng/mL with a detection limit of 0.7ng/mL. A direct competitive ELISA using an enzyme tracer was also developed, which showed an IC"5"0 of 6.0ng/mL with a detection limit of 0.9ng/mL. The antibodies in both assays showed negligible cross-reactivity with metabolites of diazinon and other OP pesticides. Recovery of diazinon from fortified lettuce and rice samples was satisfactory except at the fortified concentration of 100ppb.