Enzyme-Linked Immunosorbent

A rapid and sensitive chemiluminescence enzyme-linked immunosorbent assay for the determination of fumonisin B"1 in food samples [An article from: Analytica Chimica Acta]
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
An enzyme-linked immunosorbent assay (ELISA) based on polyclonal antibody with enhanced chemiluminescent (ECL) detection of fumonisin B"1 (FB"1) in food samples has been developed. Assay conditions, including concentrations of antibody and enzyme conjugate, competition time and so on, were optimized. The effects of pH and two different organic solvents were investigated. The optimized ECL-ELISA system allowed FB"1 determination in a linear working range of 0.14-0.9@mgL^-^1 with IC"5"0 value of 0.32@mgL^-^1 and a limit of detection of 0.09@mgL^-^1. The ECL-ELISA was about 10 times more sensitive and about 30% time less than that of colorimetric ELISA using the same antibody and HRP-conjugate. Good recoveries with spiked food samples were obtained, and the results correlated well with those obtained using conventional direct competition ELISA assay and HPLC method, which indicated that ECL-ELISA was capable of being applied for the specific detection and routine monitoring of FB"1 in food samples.

Multiple enzyme linked immunosorbent assay system on a capillary-assembled microchip integrating valving and immuno-reaction functions [An article from: Analytica Chimica Acta]
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Multiple enzyme linked immunosorbent assay (ELISA) chip is developed by using capillary-assembled microchip (CAs-CHIP) technique, which involves simple embedding of 2-3mm length of square capillaries possessing valving and immuno-reaction functions into the microchannels fabricated on a PDMS substrate. In contrast to the previously reported ELISA chips, our system enables not only the flexible design of the multi-ELISA chip required for many different diagnostic purposes, but also the valving operation required for a reliable analysis. Here, a thermo-responsive polymer-immobilized capillary was used together with a small Peltier device, as a valving part, and different antibody-immobilized capillaries were used as immuno-reaction part. Sample solution and detecting reagent solutions were sequentially introduced through the valving capillary, and the valve is closed to completely stop the solution flow inside the immuno-reaction capillaries and detected using thermal lens microscope (TLM). Different anti-IgGs (human, goat, chicken) were immobilized and used as ELISA parts of CAs-CHIP. Sequential introductions of the mixed IgG solution, mixed enzyme-antibody solution and substrate solution facilitated the multiple determinations of 0.1ngmL^-^1 IgGs (human, goat, chicken) with total analysis time of about 30min. The valve-integrated multi-ELISA chip developed here can be applied for many different diagnostic purposes by using different immuno-reaction capillaries necessary for a specific clinical diagnostic application.

Development of a flow-through enzyme-linked immunosorbent assay and a dipstick assay for the rapid detection of the insecticide carbaryl [An article from: Analytica Chimica Acta]
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Membrane-based competitive enzyme immunoassays in flow-through and dipstick format for the rapid detection of carbaryl were developed. A nylon membrane was coated with anti-carbaryl antibody and a carbaryl-horseradish peroxidase (HRP) conjugate was used as the labeled antigen for competitive assay of carbaryl. Both tests had visual detection limits of 10@mgL^-^1, sensitive enough to meet the requirements of regulatory agencies as a practical screening tool. Matrix interference was eliminated by appropriate dilution of sample extracts with buffer. With assay times of 5min (flow-through) and 15min (dipstick), these rapid tests can be useful as a quality control tool and can be used to qualitatively detect carbaryl.

Direct sub-ppt detection of the endocrine disruptor ethinylestradiol in water with a chemiluminescence enzyme-linked immunosorbent assay [An article from: Analytica Chimica Acta]
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
A chemiluminescence ELISA for the direct detection of ethinylestradiol (EE2) in water at sub-ppt levels was developed and validated. At a signal-to-noise ratio of three the detection limit is 0.2+/-0.1ngL^-^1, at a ratio of 10 the LOQ is found to be 1.4+/-0.8ngL^-^1. Based on a conservatively calculated precision profile the analytical working range is established from 0.8 to 100ngL^-^1. The ELISA was tested in four different matrices, including surface water and effluent of sewage treatment plants. All measurements were validated using an LC-MS/MS method. Typical results were consistent in both methods below 1ngL^-^1. Using this chemiluminescence ELISA facilitates for the first time the direct detection of EE2 at ecotoxicologically relevant concentrations.